nccit teratocarcinoma cell line  (ATCC)

Journal: Cell Death Discovery

Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

doi: 10.1038/s41420-025-02802-4

Figure Lengend Snippet: a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

Techniques: Knockdown, Expressing, Gene Expression, Over Expression

Journal: Cell Death Discovery

Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

doi: 10.1038/s41420-025-02802-4

Figure Lengend Snippet: a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Roles of transposable elements and DNA methylation in the formation of CpG islands and CpG-depleted regulatory elements

doi: 10.1073/pnas.2502963122

Figure Lengend Snippet: SINEs located near non-CGI_TSSs have limited effects on transcriptional activity. Schematic diagrams ( Left ) illustrate the luciferase reporter constructs containing the regulatory regions of OCT4 [( A ) −2601 to +53] and NANOG [( B ) −1857 to +239], with or without nearby SINE elements ( AluJ or AluSx , shown in blue). The numbers indicate positions relative to the TSS, with +1 representing the TSS; negative values indicate upstream regions, while positive values indicate downstream regions. The corresponding bar graphs ( Right ) show transcriptional activity measured by dual-luciferase reporter assays in NCCIT cells. Data represent mean ± SEM from three independent experiments (n = 3). Graphs show mean and ±SEM (n = 3, * P <0.05, ns: not statistically significant).

Article Snippet: Embryonic carcinoma NCCIT cells (American Type Culture Collection) were cultured in RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum.

Techniques: Activity Assay, Luciferase, Construct